Granuphilin c2a
WebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of … WebFeb 11, 2024 · Seventy percent of the world’s internet traffic passes through all of that fiber. That’s why Ashburn is known as Data Center Alley. The Silicon Valley of the east. The …
Granuphilin c2a
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NMR analysis of the granuphilin-C2A domain interacting with PI(4,5)P2-nanodisc demonstrates that, besides residues within the concave groove that are responsible for PI(4,5)P2 head group binding, the strand β5 was also affected. This region may be involved in direct binding to membrane and/or undergo conformation changes upon membrane binding. WebAug 24, 2024 · The C2A domain of granuphilin binds strongly to plasma membranes containing PIP2 via a conserved lysine cluster (red circle) embedded in a large electropositive surface (blue oval) containing many basic residues (blue sticks). A single exposed hydrophobic residue (gray sticks) also contributes to membrane affinity.
WebAll Slp members basically share an N-terminal Slp homology domain (SHD) and C-terminal tandem C2 domains (named the C2A domain and the C2B domain) with the SHD and C2 domains being separated by a linker sequence of various length. Slp1/JFC1 and Slp2/exophilin 4 promote granule docking to the plasma membrane. Additionally, their … WebFeb 15, 2006 · Synaptotagmin-like protein 4-a (Slp4-a)/granuphilin-a is specifically localized on dense-core vesicles in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. However, the precise molecular mechanism of its inhibitory effect on exocytosis has never been …
WebMar 7, 2002 · Granuphilin/Slp-4 is a member of the synaptotagmin-like protein family expressed in pancreatic β-cells and in the pituitary gland. We show by confocal microscopy that both granuphilin-a and -b colocalize with insulin-containing secretory granules positioned at the periphery of pancreatic β-cells. Overexpression of granuphilins in … WebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2. 7: 24184645: 2014: The C2 domains of granuphilin are high-affinity sensors for plasma membrane lipids. 5: 27032672: 2016: Granuphilin exclusively mediates functional granule docking to the plasma membrane. 4:
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WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma … high pivot suspensionWebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2 MLA Chanjuan Wan, et al. "Insights Into the Molecular Recognition of the Granuphilin C2a … high pixel digital cameraWebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this study was to determine membrane-binding mechanisms, affinities, and kinetics of both granuphilin C2 domains using fluorescence spectroscopic techniques. how many ball boys at wimbledonWebusing site-directed mutagenesis and NMR. Overall, granuphilin demonstrates the capability to bind to background anionic lipids and phosphoinositides inde-pendently, but the presence of both lipids greatly increases the binding affinity of this protein. This suggests that granuphilin may serve as a coincidence de- how many ball brothersWebNov 25, 2005 · Deletion analysis showed that the syntaxin-2/3 (or Munc18-1/2)-binding site is a linker domain of Slp4-a (amino acid residues 144-354), a previously uncharacterized region located between the N-terminal Rab27A binding domain and the C2A domain. We also found that the Slp4-a.syntaxin-2 complex is actually present in rat parotid glands … high pixel wiki all minion skinsWebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. ... high pixel port addressWebThe C2A domain plays key roles in this process through interaction with PI (4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A … high pixel server info