Fitc-conjugated pac-1

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August undefined, 2024

WebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. WebJun 1, 2002 · On human platelets, the high affinity conformation of alphaIIbbeta3 is detected by the monoclonal antibody (mAb), PAC-1. However, a reagent with binding specificity to high affinity mouse alphaIIbbeta3 has not been described so far. ... JON/A was conjugated with fluorescein isothiocyanate (JON/A(FITC)) or with R-phycoerythrin (JON/A(PE)) and ...

Platelet Activation - BD Biosciences

WebThe FITC-conjugated antibodies employed in a laboratory for the DFA test may be developed in house or commercially supplied. Some laboratories use a cocktail of monoclonal antibodies raised in mice against prominent antigens (e.g., nucleoprotein or glycoprotein), while others use a polyclonal antirabies antiserum raised in suitable … WebThe antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. ... a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue … diary of a mad cat

FITC Anti-C1q antibody (ab4223) Abcam

WebAug 1, 2003 · MFI of PAC-1-FITC was assessed in 5 whole blood samples immediately, 15, 30, and 60 min after antibody labelling with PAC-1 and CD42b MoAbs. WebFeb 16, 2024 · The blood platelets were distinguished based on the expression of CD61. For each sample, 10,000 CD61-positive objects (platelets) were acquired. For the assessment of P-selectin expression, samples were labeled with fluorescently conjugated monoclonal antibody PAC-1-FITC. Results are shown as the percentage of platelets binding PAC … WebFITC conjugates of antibodies, lectins, hormones, and growth factors have been used in a variety of immunohistochemical and flow cytometry applications. The protocols have been optimized for antibodies, but may be adapted to other proteins by the end user. diary of a mad blender summary

FITC Protocols Thermo Fisher Scientific - US

Colorectal Cancer Proteintech Group

WebKLH conjugated synthetic peptide derived from human AMPD3: Concentration - 100 μg (in 100 μl): 1 mg/ml: Form/Appearance: Lyophilized or Liquid: Conjugation: FITC: MW: 89kDa: UniProt ID: O08739, Q01432, O09178: Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. WebThe binding of fibrinogen to the gpIIb/IIIa receptor is required for platelet aggregation. PAC-1 binds only to activated platelets and appears to be … cities near asheboro ncWebNov 19, 2014 · FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2 were prepared, and their integrin αvβ3/αvβ5 binding affinity was determined using the displacement assay against (125)I-echistatin bound to U87MG glioma cells. IC50 values of FITC-Galacto-RGD2, FITC-3P-RGD2, and FITC-RGD2 were calculated to be 28 ± 8, 32 ± 7, and 89 ± 17 nM, … diary of a mad black woman watch

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Fitc-conjugated pac-1

WebPAC-1 Monoclonal Antibody (PAC-1), FITC, Invitrogen™-Mouse Monoclonal Antibody Shop PAC-1 Monoclonal Antibody ... PAC-1 Monoclonal antibody specifically detects PAC-1 in Human samples. It is validated for Flow Cytometry ... PAC-1: Classification: Monoclonal: Conjugate: FITC: Gene: EIF3EP1: Immunogen: Human platelets: Quantity: 100 Tests ... WebPac 1 Fitc Conjugate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

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Websupplied, and the antibody subclass for each FITC-conjugated antibody. The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. Antibody Concentration Amount Subclass Anti-His(C-term)-FITC 1 mg/mL 50 μl (25 reactions)† IgG 2b Anti-myc-FITC 1 mg/mL 50 μl (25 reactions)† IgG 1 Webphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and ﬂuorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA).

WebIHC-plus™ ADCYAP1R1 / PAC1 Receptor Antibody LS-A109382 is a pathologist validated IHC antibody FITC Rabbit anti-Human ADCYAP1R1 / PAC1 Receptor. Validated for IHC. Tested on 20 paraffin-embedded human tissues. ... Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.) Purification. WebProduct Specific Information. The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on …

WebFluorescein Isothiocyanate (FITC)-conjugated phal- loidin was obtained from Invitrogen (Invitrogen, Carlsbad, CA, USA). Alexa 488-conjugated anti-rabbit anti-body and 4′,6-diamidino-2-phe- nylindole (DAPI) were obtained from Invitrogen. HPLC solvents were purchased from Fisher Scientific Ltd (Korea). General experimental procedures WebData on immunogenicity of adenovirus-vectored vaccine in chronic obstructive pulmonary disease (COPD) patients is limited. Therefore, we aimed to determine the humoral and cellular immune responses after homologous ChAdOx-1 vaccination in subjects with COPD. COPD subjects and age- and sex-matched healthy elderly receiving ChAdOx-1 …

WebMutated KRAS proteins are often constitutively active, resulting in the dysregulated growth and transformation pathways that promote pancreatic cancer growth, survival, and metastasis. Proteintech’s KRAS antibody is a favorite among researchers worldwide, cited in nearly 100 publications. Immunohistochemical analysis of paraffin-embedded ...

WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation. diary of a mad cat mrs katswellWebColorectal cancer (CRC) is the third most common form of cancer worldwide, with men having a slightly higher incidence rate compared to women. Risk for developing colorectal cancer usually increases with age and can be attributed to inherited genetic mutations along with other comorbidities such as diabetes, IBD, obesity, poor diet, and an ... cities near asbury park njWebApr 4, 2024 · PAC-1 Antibody (4O21) is available as both the non-conjugated anti-PAC-1 antibody form, as well as multiple conjugated forms of anti-PAC-1 antibody, including agarose, HRP, PE, FITC and … diary of a mad divaWebIn Fig. 1a, we used FITC- conjugated anti-human fibrinogen antibody to highlight intercellular reactivity (white dots, red arrow). The round structures (blue arrow) seem to be a series of TTS ... cities near arlington vaWebThe PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets. The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, α IIb β 3) complex of activated platelets at or near the platelet fibrinogen receptor. cities near athens georgiaWebApr 7, 2024 · And then, they were thoroughly washed, stained orderly with the vinculin antibody (1:500) overnight at 4 °C, the FITC-conjugated goat-anti-rabbit secondary antibody (1:5000) for 1 h, rhodamine-conjugated phalloidin (1:250) for 1 h, and DAPI (1:5000) for 5 min at room temperature. cities near atlantic cityWebBecton Dickinson fluorescein isothiocyanate fitc conjugated pac 1 antibody Fluorescein Isothiocyanate Fitc Conjugated Pac 1 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... cities near athens ga